pct-100 programmable thermal controller (MJ Research)
90
Structured Review
MJ Research
pct-100 programmable thermal controller
Pct 100 Programmable Thermal Controller, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pct-100+programmable+thermal+controller/pct+100+programmable+thermal+controller/pmc11331963-144-42-46
Average 90 stars, based on 1 article reviews
Pct 100 Programmable Thermal Controller, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pct-100+programmable+thermal+controller/pct+100+programmable+thermal+controller/pmc11331963-144-42-46
Average 90 stars, based on 1 article reviews
pct-100 programmable thermal controller - by Bioz Stars,
2026-10
90/100 stars
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Polymerase Chain Reaction:Article Title: Novel Light-Upon-Extension Real-Time PCR Assays for Detection and Quantification of Genogroup I and II Noroviruses in Clinical Specimens Article Snippet: .. For the optimization, a final volume of 25 μl of reaction mixture containing 2.5 μl cDNA from the RT reaction, 2.5 μl of 10× PCR buffer, 1 μl of MgCl 2 (50 mM), 2 μl of deoxynucleoside triphosphates (2.5 mM), 0.5 μl of each primer (10 pmol μl −1 ) (Table ), 0.125 μl of Taq polymerase (5 U μl −1 ) (Invitrogen, Carlsbad, CA), and 15.875 μl of RNase-free water was used, and the reaction was carried out on a PCT-100 programmable Article Title: Novel Light-Upon-Extension Real-Time PCR Assays for Detection and Quantification of Genogroup I and II Noroviruses in Clinical Specimens Article Snippet: .. For the optimization, a final volume of 25 l of reaction mixture containing 2.5 l cDNA from the RT reaction, 2.5 l of 10 PCR buffer, 1 l of MgCl2 (50 mM), 2 l of deoxynucleoside triphosphates (2.5 mM), 0.5 l of each primer (10 pmol l 1) (Table 1), 0.125 l of Taq polymerase (5 U l 1) (Invitrogen, Carlsbad, CA), and 15.875 l of RNase-free water was used, and the reaction was carried out on a PCT-100 programmable Article Title: Sixteen EST-linked microsatellite markers in Günther's walking catfish, Clarias macrocephalus. Article Snippet: Twenty-seven new microsatellite sequences were identified by screening 2029 expressed sequence tags from Günther’s walking catfish, Clarias macrocephalus.. Sixteen loci were polymorphic with the number of alleles ranging from two to 16 per locus and the observed and expected heterozygosities ranging from 0.4667 to 0.9333 and from 0.427 to 0.8819 per locus, respectively.. Cross-species amplifications of all 16 primer pairs were tested in four other species of catfish including Clarias gariepinus, Pangasius hypophthalmus, Pangasius larnaudii and Pangasianodon gigas. Article Title: Novel Light-Upon-Extension Real-Time PCR Assay for Simultaneous Detection, Quantification, and Genogrouping of Group A Rotavirus Article Snippet: .. The PCR mixture contained 2.5 l cDNA from the RT reaction, 2.5 l of 10 PCR buffer, 1 l of 50 mM MgCl2, 2 l of 2.5 mM deoxynucleotide triphosphate (dNTP), 0.5 l of 10 pmol l 1 of each primer, 0.125 l of 5 U l 1 Taq polymerase (Invitrogen, Carlsbad, CA), and RNase-free water up to a final volume of 25 l. The PCR was carried out on a PCT-100 programmable Article Title: Development of PCR-Based Markers Linked to Quantitative Resistance to Late Blight in a Diploid Hybrid Potato Population of Solanum phureja x S. stenotomum Article Snippet: A diploid hybrid Solanum phureja x S. stenotomum population that has shown relatively high levels of quantitative resistance to late blight was used to develop polymerase chain reaction (PCR)-based markers for marker-assisted selection (MAS) of disease resistance in potato breeding.. Two random amplified polymorphic DNA (RAPD) markers, OPA03576 and OPA17559, were identified as being linked to partial resistance.. OPA03576 was found to be associated in coupling phase while OPA17559 showed association in repulsion phase to resistance. Article Title: Novel Light-Upon-Extension Real-Time PCR Assay for Simultaneous Detection, Quantification, and Genogrouping of Group A Rotavirus Article Snippet: The PCR mixture contained 2.5 μl cDNA from the RT reaction, 2.5 μl of 10× PCR buffer, 1 μl of 50 mM MgCl 2 , 2 μl of 2.5 mM deoxynucleotide triphosphate (dNTP), 0.5 μl of 10 pmol μl −1 of each primer, 0.125 μl of 5 U μl −1 Taq polymerase (Invitrogen, Carlsbad, CA), and RNase-free water up to a final volume of 25 μl. .. The PCR was carried out on a PCT-100 programmable Concentration Assay:Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate. Article Snippet: The aim of this study was to determine the effect of oligosaccharide extract from bamboo shoot (BOS) on the performance, intestinal morphology, microbiota and immune reaction of laying hens challenged with dextran sodium sulfate (DSS).. Thirty-two White Leghorn hens (480 days old) were divided into 4 groups (8 hens each) with similar mean body weights: C (basal diet), D (basal diet + DSS), B (5 g/kg BOS diet), and BD (5 g/kg BOS diet + DSS).. They were administered a single oral dose of 4 mL of distilled water/kg body weight with or without 0.45 g of DSS for 7 consecutive days from the 14th d to the 21st d of the experiment. Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate Article Snippet: For intestinal tissues, total RNA was extracted and purified using a NucleoSpin RNA Kit (Macherey-Nagel GmbH & Co. KG., Duren, Germany) according to the manufacturer's instructions and then stored at −80°C until analysis. .. The total RNA concentration was measured using a NanoDrop Lite (Thermo Fisher Scientific, MA, USA). cDNA was produced from the total RNA by reverse transcription using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PCT-100 programmable Produced:Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate. Article Snippet: The aim of this study was to determine the effect of oligosaccharide extract from bamboo shoot (BOS) on the performance, intestinal morphology, microbiota and immune reaction of laying hens challenged with dextran sodium sulfate (DSS).. Thirty-two White Leghorn hens (480 days old) were divided into 4 groups (8 hens each) with similar mean body weights: C (basal diet), D (basal diet + DSS), B (5 g/kg BOS diet), and BD (5 g/kg BOS diet + DSS).. They were administered a single oral dose of 4 mL of distilled water/kg body weight with or without 0.45 g of DSS for 7 consecutive days from the 14th d to the 21st d of the experiment. Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate Article Snippet: For intestinal tissues, total RNA was extracted and purified using a NucleoSpin RNA Kit (Macherey-Nagel GmbH & Co. KG., Duren, Germany) according to the manufacturer's instructions and then stored at −80°C until analysis. .. The total RNA concentration was measured using a NanoDrop Lite (Thermo Fisher Scientific, MA, USA). cDNA was produced from the total RNA by reverse transcription using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PCT-100 programmable Reverse Transcription:Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate. Article Snippet: The aim of this study was to determine the effect of oligosaccharide extract from bamboo shoot (BOS) on the performance, intestinal morphology, microbiota and immune reaction of laying hens challenged with dextran sodium sulfate (DSS).. Thirty-two White Leghorn hens (480 days old) were divided into 4 groups (8 hens each) with similar mean body weights: C (basal diet), D (basal diet + DSS), B (5 g/kg BOS diet), and BD (5 g/kg BOS diet + DSS).. They were administered a single oral dose of 4 mL of distilled water/kg body weight with or without 0.45 g of DSS for 7 consecutive days from the 14th d to the 21st d of the experiment. Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate Article Snippet: For intestinal tissues, total RNA was extracted and purified using a NucleoSpin RNA Kit (Macherey-Nagel GmbH & Co. KG., Duren, Germany) according to the manufacturer's instructions and then stored at −80°C until analysis. .. The total RNA concentration was measured using a NanoDrop Lite (Thermo Fisher Scientific, MA, USA). cDNA was produced from the total RNA by reverse transcription using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PCT-100 programmable Real-time Polymerase Chain Reaction:Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate. Article Snippet: The aim of this study was to determine the effect of oligosaccharide extract from bamboo shoot (BOS) on the performance, intestinal morphology, microbiota and immune reaction of laying hens challenged with dextran sodium sulfate (DSS).. Thirty-two White Leghorn hens (480 days old) were divided into 4 groups (8 hens each) with similar mean body weights: C (basal diet), D (basal diet + DSS), B (5 g/kg BOS diet), and BD (5 g/kg BOS diet + DSS).. They were administered a single oral dose of 4 mL of distilled water/kg body weight with or without 0.45 g of DSS for 7 consecutive days from the 14th d to the 21st d of the experiment. Article Title: Effects of oligosaccharides on performance, intestinal morphology, microbiota and immune reactions in laying hens challenged with dextran sodium sulfate Article Snippet: For intestinal tissues, total RNA was extracted and purified using a NucleoSpin RNA Kit (Macherey-Nagel GmbH & Co. KG., Duren, Germany) according to the manufacturer's instructions and then stored at −80°C until analysis. .. The total RNA concentration was measured using a NanoDrop Lite (Thermo Fisher Scientific, MA, USA). cDNA was produced from the total RNA by reverse transcription using ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo Co., Ltd., Osaka, Japan) on a PCT-100 programmable Amplification:Article Title: Development of PCR-Based Markers Linked to Quantitative Resistance to Late Blight in a Diploid Hybrid Potato Population of Solanum phureja x S. stenotomum Article Snippet: A diploid hybrid Solanum phureja x S. stenotomum population that has shown relatively high levels of quantitative resistance to late blight was used to develop polymerase chain reaction (PCR)-based markers for marker-assisted selection (MAS) of disease resistance in potato breeding.. Two random amplified polymorphic DNA (RAPD) markers, OPA03576 and OPA17559, were identified as being linked to partial resistance.. OPA03576 was found to be associated in coupling phase while OPA17559 showed association in repulsion phase to resistance. |